3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone. Depositing Lab: Jason Sheltzer. Publication: Giuliano et al Curr Protoc Mol Biol ... |
Full Sequences from Addgene (1). Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences ... |
Originally described in: Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Giuliano CJ, Lin A, Girish V, Sheltzer JM Curr ... |
Sequencing Result #124774. Sequence Analyzer: pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W Sequencing Result. Download: GenBank File · SnapGene File; File Help. Map ... |
Purpose. 124774, Lenti-Cas9-gRNA-TagBFP2, 3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone. 124773, LRG2.1-TagBFP2, Lentiviral ... |
#124774. Purpose. 3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone. Depositor. Jason Sheltzer. Article. |
124774, Lenti-Cas9-gRNA-TagBFP2, Mammalian Expression, Lentiviral, CRISPR, Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9 ... |
Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. |
3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection. Depositor. |
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