For example, the activity of an enzyme that converts substrate A to product B can be coupled to the activity of another enzyme that converts product B to a ... |
For example, figure 1 shows the coupled assay for the enzyme hexokinase, which can be assayed by coupling its production of glucose-6-phosphate to NADPH ... |
None of the reactants or products can be measured conveniently. By using coupled assay, glucose 6-phosphate (the product of enzyme catalyzed reaction) is made ... |
The classic continuous coupled-enzyme assay is the pyruvate kinase and lactate dehydrogenase system (Kornberg and Pricer, 1951). |
For example, an enzymatic assay of ethyl alcohol (alcohol) utilizes alcohol dehydrogenase enzyme to oxidize ethyl alcohol into acetaldehyde. |
We present a simple, quantitative, real-time method to detect the production of fumarate from succinate by complex II that is easy to implement and applicable. |
28 июн. 2021 г. · Most enzyme assays are based on spectroscopic techniques, with the two dominant types being absorption and fluorescence. |
Coupled enzyme assay is a robust, non-radioactive, continuous assay ... For example, an enzymatic assay of l-malic acid uses an NAD(P)-linked malic enzyme ... |
The various types of assays and some examples of using such data to characterize a pure protein and to quantitate active protein are discussed below. |
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