23 мар. 2017 г. · Here we describe a protocol for genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system.

7 дек. 2023 г. · CRISPR-screen · Mix gently with a shaker for 10 min at 25°C. · Incubate the cells at 37°C, 5% CO2 for 24 h, change the media, and add puromycin.

30 мар. 2023 г. · We present a protocol for performing pooled CRISPR-Cas9 loss-of-function screens to identify genetic modifiers using either fluorescence-based or cell death ...

Here we describe a protocol for genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system. Custom- or ready-made guide RNA ...

1. · STEP 1: select the phenotypic change you wish to study · STEP 2: choose the cells you want to use for the screen · STEP 3: express Cas9 in your target cells.

10 февр. 2022 г. · For both genome-scale and focused CRISPR knockout (CRISPRko) screens, it is advisable to include at least four different gRNAs for each target ...

15 мар. 2024 г. · This protocol uses efficient genome-editing techniques to explore macrophage involvement in immune response, chronic inflammation, neurodegenerative disease, ...

For drug treatment screens, apply the drug approximately 1 week after the initial library infection to allow sufficient time for Cas9-mediated genome editing ...

2 авг. 2022 г. · We present a protocol to conduct a genome-scale CRISPR screen on HeLa cells for host factors involved in the toxin action of Clostridioides difficile TcdB4.

Pooled Genome-Scale CRISPR-Cas9 Knock-out Screens in Human Cells. This is a research protocol that describes a protocol to perform pooled genome scale gRNA.