17 авг. 2023 г. · This protocol allows the amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to millions of different plasmids ...

30 мар. 2023 г. · We present a protocol for performing pooled CRISPR-Cas9 loss-of-function screens to identify genetic modifiers using either fluorescence-based or cell death ... Summary · Before you begin · Step-by-step method details

1. · STEP 1: select the phenotypic change you wish to study · STEP 2: choose the cells you want to use for the screen · STEP 3: express Cas9 in your target cells.

7 дек. 2023 г. · Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of ferroptosis.

In this protocol, pooled sgRNA libraries targeting thousands of genes are computationally designed, generated using microarray-based synthesis techniques, ...

23 мар. 2017 г. · We describe protocols for designing and cloning an sgRNA library, packaging lentiviral vectors for transduction, analyzing screening results, ...

Here, we describe a protocol for genome-scale CRISPR screening for use in genetically engineered human pluripotent stem cells (hPSCs).

Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in ...

25 авг. 2022 г. · Here, we detail the steps for dual-gRNA library construction, with the introduction of two backbones, LentiGuide_DKO and LentiCRISPR_DKO.

8 мар. 2023 г. · The protocols included provide the instructions on how to package the plasmid form of a pooled sgRNA library into viral particles, perform a ...