In a de novo genome assembly and annotation project, the nucleotide sequence of a genome is first assembled, as completely as possible, and then annotated.

24 янв. 2022 г. · We present a comprehensive overview of de novo transcriptome assembly and annotation. We discuss the procedures involved, including pre- and post-processing ... Abstract · Introduction · De novo transcriptome assembly

27 мая 2023 г. · The transcriptome was functionally annotated by performing DIAMOND and InterProScan. By selecting the best hit for each database, the annotation ...

22 февр. 2022 г. · It uses a repeat graph as its core data structure and utilises raw data in FASTA or FASTQ format from PacBio®. Flye outputs polished contigs ...

The article gives a detailed overview of de novo genome assembly, including workflow, quality assessment, and polising.

In this protocol we discuss and outline the process of de novo assembly for small to medium sized genomes.

The resultant genome assemblies were used for repetitive sequences and transposable elements (TEs) annotation. We adopted two strategies, de novo identification ... Data Report · Introduction · Methods · Code Availability

26 сент. 2023 г. · The annotated genome and transcriptome will be a valuable resource that will allow characterization of the genetic basis of complex traits in ...

Here I introduce a protocol for the isolation, sequencing, and taxonomic classification of potentially novel species of culturable bacteria.

How do I annotate my genome? Learning Objectives: Understand what de novo genome assembly is and how it differs from reference-based assembly. Assemble ...