Hence cloning by ET recombination resembles PCR in that both involve the amplification of a DNA region between two chosen points. We apply the strategy to ...

Homologous recombination is the exchange of sequences that occurs between two similar (homologous) nucleic acid molecules. This happens in both prokaryotes ...

16 мар. 2015 г. · We tested critical parameters influencing the efficiency of PCR fragments cloning into PCR-amplified vectors by homologous recombination in the widely used E. ...

15 дек. 2016 г. · The lambda red system is an alternative method that can be used for cloning or genome engineering and is based on homologous recombination.

Recombineering is a powerful technique because it enables cloning of DNA fragments independent of restriction enzymes or PCR-based manipulation of the DNA ...

3 июн. 2015 г. · Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain.

Cloning by homologous recombination (HR) in Saccharomyces cerevisiae is an extremely efficient and cost-effective alternative to other methods of recombinant ...

16 янв. 2024 г. · For cloning by retrieval, the investigator must first add the terminal homologies necessary for recombination to the plasmid backbone. This is ...

Hence cloning by ET recombination resembles PCR in that both involve the amplification of a DNA region between two chosen points. We apply the strategy to ...

28 окт. 2015 г. · Abstract. Background: The homologous recombination (HR) is one of the conventional cloning methods for the production of recombinant DNA.