Homologous recombination is the exchange of sequences that occurs between two similar (homologous) nucleic acid molecules. This happens in both prokaryotes ...

Homologous recombination is a fundamental biological process. Biochemical understanding of this process is most advanced for Escherichia coli.

Hence cloning by ET recombination resembles PCR in that both involve the amplification of a DNA region between two chosen points. We apply the strategy to ...

A minimum of approximately equal to 20 base pairs in a completely homologous segment is required for significant recombination. There is an exponential increase ...

28 мар. 2022 г. · Homologous recombination (HR) is critically important for chromosomal replication, as well as DNA damage repair in all life forms.

A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA.

Sometime during the life cycle of virtually every organism, homologous chromosomes pair and exchange parts to produce recombinant chromosomes.

24 мая 2020 г. · The widely used methods for genome editing in E. coli were developed on the basis of λ-Red promoted homology-dependent recombination (HDR).

PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli.

We explored the use of recE-mediated homologous recombination to generate molecular diversity in Escherichia coli. Two homologous genes were placed on different ...