θ=1.22λD θ = 1.22 λ D , where λ is the wavelength of light (or other electromagnetic radiation) and D is the diameter of the aperture, lens, mirror, etc., with which the two objects are observed. In this expression, θ has units of radians.
The NA for a lens is NA = n sinα, where n is the index of refraction of the medium between the objective lens and the object at point P. From this definition ... |
The Rayleigh criterion stated in the equation θ=1.22λD θ = 1.22 λ D gives the smallest possible angle θ between point sources, or the best obtainable ... |
Limit of resolution is given by,. Limit of resolution =d=0.61λNA=0.61λμsinα. where NA=Numerical Aperture of the microscope,. μ= Refractive index of the medium,. |
In some instances, such as confocal and fluorescence microscopy, the resolution may actually exceed the limits placed by any one of these three equations. |
If all aberrations and distortions are eliminated from the optical system, this will be the limit to resolution. If aberrations and distortions are present, ... |
The theoretical maximum optical resolution of optics is determined by diffraction effects of light which mainly depend on the wavelength of the light and the ... |
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