NEB's restriction enzyme buffer system makes your restriction digests easy and convenient. We are able to offer >210 restriction enzymes that cut in a single ... |
By definition, 1 unit of restriction enzyme will completely digest 1 μg of substrate DNA in a 50 μl reaction in 60 minutes. This enzyme : DNA : reaction volume ... |
How stable are restriction enzymes? A. All restriction enzymes from NEB are assayed for activity every 3–6 months. Most are very stable when stored at -20°C ... |
19 мая 2022 г. · Samples are processed within 48 h. The purified DNA is 20–25 kb in size and can be digested by restriction enzymes. The soil is vortex-mixed ... |
Videolar I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the ... |
27 мая 2016 г. · I have used a combination of NEB and FastDigest Enzymes: it seems to work fine for me, in fact well I would say. |
In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through ... |
In general, we recommend 5–10 units of enzyme per µg DNA, and 10–20 units for genomic DNA in a 1 hour digest. DNA: Should be free of contaminants such as phenol ... |
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