18 янв. 2016 г. · By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ...

We use recently devised sgRNA design rules to create human and mouse genome-wide libraries, perform positive and negative selection screens.

22 окт. 2024 г. · Here, we use recently devised sgRNA design rules to create human and mouse genome-wide libraries, perform positive and negative selection ...

We use recently devised sgRNA design rules to create human and mouse genome-wide libraries, perform positive and negative selection screens.

Recently devised sgRNA design rules are used to create human and mouse genome-wide libraries, perform positive and negative selection screens.

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF ...

CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to ...

Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and ...

1 февр. 2016 г. · CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), ...

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. https://doi.org/10.1038/nbt.3437 · Full text. Journal: Nature ...