18 янв. 2017 г. · CROP-seq enables pooled CRISPR screens for complex transcriptome signatures by making gRNA expression detectable in single-cell RNA ...

Here we combine pooled CRISPR screening with single-cell RNA sequencing into a broadly applicable workflow, directly linking guide RNA expression to ...

27 окт. 2016 г. · Pooled CRISPR screening is a powerful and widely used method for identifying critical genes involved in biolog- ical mechanisms such as cell ...

This approach allowed us to link guide-RNA expression to the associated transcriptome responses in thousands of single cells using a straightforward and broadly ...

27 окт. 2016 г. · Abstract. CRISPR-based genetic screens have revolutionized the search for new gene functions and biological mechanisms.

We combined pooled CRISPR screening with single-cell RNA sequencing into a broadly applicable workflow, directly linking guide RNA expression to transcriptome ...

CROP-seq enables pooled CRISPR screens for complex transcriptome signatures by making gRNA expression detectable in single-cell RNA sequencing.

CRISPR screening followed by single-cell analysis (CRISPRsc) provides critical gene information on how cellular subpopulations will respond to any treatment.

9 апр. 2021 г. · Here, we present a protocol to perform pooled CRISPR-activation screens in mouse embryonic stem cells using 10× Genomics scRNA-seq as a readout.

10 февр. 2022 г. · In pooled CRISPR screens, various genetically encoded perturbations are introduced into pools of cells. The targeted cells proliferate under a ...