Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.

This tool calculates the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases. How to use this calculator.

The PCRBIO Tm Calculator is designed to estimate a suitable annealing temperature when using PCR and qPCR products from PCR Biosystems. To use the Tm calculator ...

The most sophisticated Tm calculations take into account the exact sequence and base stacking parameters, not just the base composition(1,2,3).

The melting temperature calculations are based on the thermodynamic relationship between entropy, enthalpy, free energy and temperature.

This tool calculates the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases. How to use this calculator.

This software can calculate the optimum PCR annealing temperature (Tm value) of the primer. 1. Enter the primer sequence in the textbox for which the Tm ...

Basic Tm Degenerated nucleotides are allowed. Base-Stacking Tm Degenerated nucleotides are NOT allowed. Primer concentration: nM. Salt concentration:

OligoAnalyzer is a primer analysis tool for oligonucleotides. Design and analyze DNA and RNA oligos for insight into behavior and properties.

TM Calculator. This application calculates the Tm for a primer, and gives instructions on how to dilute the primer to a desired concentration.