Primer design and other tools. Design your In-Fusion primers with our step-by-step design tool, or access the molar ratio calculator and construct simulator.

Calculate the Tm value using the following method: *Tm Calculation formula: Tm (°C) = 2 (NA + NT) + 4 (NC + NG) -5. Use this method only for a primer <25 bases.

Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.

This tool calculates the T m of primers and estimates an appropriate annealing temperature when using different DNA polymerases.

When the Tm value is 55℃ or less, set the annealing temperature to 55℃. ＊ Tm value calculation method: Tm (℃ ) = [(the number of A and T) x 2] + [(the number ...

27 дек. 2017 г. · Generally New England Biolab can give you good annealing temp prediction. -5C of Tm for annealing temp works fine.

Annealing temperature Try 55℃ first. • Annealing time. If primer Tm＊ is ≧55℃, set to 5 sec. If primer Tm＊ is ＜ 55℃, set to 15 sec. ＊ : How to calculate Tm: Tm ...

This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. NEB calculator · Dilution Calculator · Using the Ligation module · Mass ⇄ Moles

Toyobo is a leading manufacturer of enzymes and reagents for life science research and industrial fields based on our original technologies about PCR enzymes, ... PCR · Technology · High Speed & Efficient PCR... · Highly Functional PCR enzyme

This software can calculate the optimum PCR annealing temperature (Tm value) of the primer. 1. Enter the primer sequence in the textbox for which the Tm ...