Оценка 5,0 (1) However, if the temperature is too high, it will cause the enzyme to denature. These results show that the enzyme worked best in the 40-45°C range. This was ... |
Temperature 40”. * No clot occurred after adjustment to pH 5.6. The protein-free trypsin described in the preceding paper (2) was used in similar experiments, ... |
2. In a cuvette, measure 2ml of milk using a 2ml micropipette and place into the device's cavity. 3. Measure 1ml of 1% trypsin using a micropipette set aside. |
Temperature – the rate of reaction of enzymes varies with temperature. As ... Add 2 cm3 of trypsin solution to the milk in the cuvette. Working quickly ... |
4. Stand the test tube containing the solution of milk powder and the test tube containing trypsin and buffer in a water bath at 20 C. 5. Leave both tubes in ... |
Enzymes have specific functions, which can be affected by factors including temperature, pH, the presence of inhibitors, and the concentrations of reactants ... |
2. Take three test tubes and measure 5cm3 milk into each. Place in water bath at 10°C for 5 minutes to equilibrate. |
Now remove the milk test tube and the trypsin test tube from the 20°C water bath and add the contents of each to a single cuvette, shaking it and then ... |
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