The orthodox type II restriction endonuclease is a homodimer of ∼2 × 30 kDa molecular mass, which recognizes a palindromic sequence 4–8 bp in length, and in the presence of Mg 2 + cleaves the two strands of the DNA within or immediately adjacent to the recognition site to give a 5′-phosphate and a 3′-OH end.
Type II restriction enzymes are the familiar ones used for everyday molecular biology applications such as gene cloning and DNA fragmentation and analysis. |
Type II restriction endonucleases are components of restriction modification systems that protect bacteria and archaea against invading foreign DNA. |
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A Class II restriction endonuclease recognizes a specific sequence and cuts at a specific sequence. A Class II methylase is site specific and transfers the ... |
Type II restriction endonucleases always cleave at or near their recognition sites. They produce small, well-defined fragments of DNA that help to characterize ... |
Restriction endonucleases are prokaryotic enzymes which function to protect the host cell from the invasion of foreign DNA [1]. They function as components ... |
These site-specific deoxyribonucleases catalyse the endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. |
Here we discuss 'Type II' REases, the kind used for DNA analysis and cloning. We focus on their biochemistry: what they are, what they do, and how they do it. |
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