25 июн. 2021 г. · It is generally suggested to maintain your DNA concentration around 25 ng/μL for qPCR (but 20 to 30 ng/μLcan be used).

Good-quality DNA will have an A260/A280 ratio of 1.7–2.0. A reading of 1.6 does not render the DNA unsuitable for any application, but lower ratios indicate ...

Suppliers of MVS instruments define the detection limits between 0.5 to 2 ng/µl, but warn that below 20 ng/µl the reliability of the purity ratios is ...

Tamari et al. (2013) reported that DNA isolated from matured and apical leave have good concentration than other tissues ranging from 250-525 and 650-1125 ...

The effective read range of UV spectroscopy is 0.1 to 0.999 which corresponds to approximately 4 ng/µL to 50 ng/µL of genomic DNA. Values above or below that ...

As little as 20 ng DNA can be detected ... In most experiments the effective range for comparative densitometric quantification is between 20 and 100 ng.

The best DNA profile was obtained from samples with DNA concentration at 0.5 – 1.8 ng/25 μL. Reliable DNA profiles were obtained from samples with 0.4, 0.5 and ...

29 мая 2024 г. · In this case, your sample would have a concentration of around 8 ng/µl (mass of DNA fragments of the DNA ladder divided by sample volume = 40 ng ...

22 апр. 2022 г. · 100-300 ng/ul (roughly) has tended to be the sweet spot in my experience. Not all samples are that concentrated, and you can definitely measure ...